Androcare Fertility Laboratories and Cryobank

Sex Selection

ABOUT OUR SEX SELECTION TECHNOLOGY

Our sex selection method is through MicroSort which  is a scientifically proven preconception process that increases the probability of having a baby of the desired gender.

Semen samples usually contain 50% of spermatozoa carrying the Y chromosome that will produce a boy and a 50% of spermatozoa carrying the X chromosome that will produce a girl.

The MicroSort process separates spermatozoa, the ones that will result in a girl or the ones that will result in a boy, from the rest of the sample, allowing a couple to use a sample that only contains the spermatozoa that was selected according to the criteria we choose: for a healthy boy or a healthy girl.

The samples processed by MicroSort can be used in a wide variety of artificial reproduction techniques, such as intrauterine insemination (IUI), in vitro fertilization, intracytoplasmic sperm injection, preimplantation genetic diagnosis, among other procedures.

REQUIREMENT FOR SEX SELECTION

Couples who want to use MicroSort to balance the genders of their children must:

•         Be married

•         Be negative for HIV, and Hepatitis B surface antigen and C antibody

•         Sperm count of 20million and above with a percentage motility of 50%

What are the requirements for couples at risk for X-linked disorders?

Couples who want to use MicroSort to reduce the probability of genetic disease transmission must:

•         Be married

•         Be a known carrier of an X-linked or X-limited disorder

•         Be negative for HIV, and Hepatitis B surface antigen and C antibody

•         Abstinence of 2 or 3 days.

•         No alcohol consumption over the abstinence period.

•         No exposure to high temperatures such as hot water, hot tubs, vapor rooms, practicing cycling, etc.

*Patients that have received hepatitis vaccines should get the appropriate tests to avoid false positives.

 

THE PROCESS

MicroSort is a method based on the amount difference in the amount of genetic material in the sperm, where the X chromosome has approximately 2.8% more DNA material than the Y chromosome.

During the MicroSort process the sperm sample is washed to remove seminal liquid along with not moving (nonmotile) cells. Afterwards the sample is stained with a special fluorescent stain that attaches temporarily to the DNA contained in the spermatozoa.

The stained spermatozoa are analyzed one by one by a flow cytometer, where cells go through a laser that makes the stain attached to the DNA fluoresce. The spermatozoa containing the X chromosome (which have more DNA and therefore more stain) will have a bigger shine than the spermatozoa containing the Y chromosome.

The flow cytometer uses special software to identify sperm which exhibit the fluorescence signature of cells that match healthy sperm of the desired gender. Those sperm will be separated from the rest of the sample, obtaining an enriched sperm sample ready for use.

SUCCESS STORIES

MicroSort is a scientifically proven method, this means that results gathered to date show good fertilization, cleavage and pregnancy rates as well as constant testing that show the recovery of high purity samples.

Purity in sorted samples refers to the percentage of sperm cells that correspond to the desired gender. This purity is obtained by taking a very small part of each sorted sample and performing a fluorescence in situ hybridization (FISH) test. During this test special colored probes are attached to the specific chromosomes in the sperm, obtaining green signals for the Y chromosome carrying cells and red signals for the X chromosome bearing cells; these signals are counted and a percentage is obtained. This shows the percentage of cells of the desired gender in sorted samples.

The purity percentages for MicroSort sorted samples will vary according to different factors such as the amount of motile cells, progression, difference in amount of DNA between X and Y chromosome (this changes slightly according to each patient), the desired gender, among others. The average purity for MicroSort samples is 91% for girls and 74% for boys.

Another form of proving that MicroSort is effective is in the number of babies born of the desired gender from this technology that raises the percentages to 93% for girls and 82% for boys.

With these results we can understand that MicroSort does not guarantee a baby of the desired gender, but this technology has scientifically proven to significantly increase the percentage of cells needed to produce the desired gender in a sample and therefore significantly increases the possibility to conceive a baby of the desired gender.

Our sex selection method is through MicroSort which  is a scientifically proven preconception process that increases the probability of having a baby of the desired gender.

Semen samples usually contain 50% of spermatozoa carrying the Y chromosome that will produce a boy and a 50% of spermatozoa carrying the X chromosome that will produce a girl.

The MicroSort process separates spermatozoa, the ones that will result in a girl or the ones that will result in a boy, from the rest of the sample, allowing a couple to use a sample that only contains the spermatozoa that was selected according to the criteria we choose: for a healthy boy or a healthy girl.

The samples processed by MicroSort can be used in a wide variety of artificial reproduction techniques, such as intrauterine insemination (IUI), in vitro fertilization, intracytoplasmic sperm injection, preimplantation genetic diagnosis, among other procedures.